What is CTAB Extraction Buffer used for?

CTAB Extraction Buffer is primarily used for the extraction of nucleic acids from various plant and microbial samples. It contains a cationic detergent that helps lyse cells and neutralize nucleases, ensuring the integrity of the DNA or RNA being extracted. This buffer is particularly effective when working with challenging samples like seeds or plant tissues, which often require additional support to break down cellular structures.

How do I use CTAB Extraction Buffer for DNA extraction?

To use CTAB Extraction Buffer for DNA extraction, mix the buffer with your sample in a tube, then incubate at a controlled temperature to facilitate cell lysis. After incubation, add chloroform to separate the DNA from proteins and other cellular debris. Finally, precipitate the DNA with isopropanol, followed by washing with ethanol. This method is ideal for isolating high-quality DNA necessary for downstream applications such as PCR and sequencing.

Can CTAB Extraction Buffer be used for RNA extraction?

CTAB Extraction Buffer is not typically recommended for RNA extraction, as it can introduce contaminants that inhibit downstream applications. While it can help release RNA from cells, the presence of CTAB may lead to the formation of complexes that degrade RNA quality. For RNA extraction, specific buffers optimized for that purpose are advised to yield high-purity RNA suitable for analysis.

Is CTAB Extraction Buffer suitable for bacterial samples?

Yes, CTAB Extraction Buffer works well for bacterial samples. Its cationic properties assist in lysing bacterial cell walls, allowing for the efficient extraction of DNA. When extracting from bacteria, it is essential to optimize the protocol by adjusting the incubation time and temperature, ensuring that the cell membranes are thoroughly disrupted without excessive degradation of the DNA.

What sample types can I use with CTAB Extraction Buffer?

CTAB Extraction Buffer can be effectively used with various sample types, including plant leaves, seeds, fungi, and certain bacteria. When using this buffer, it is crucial to consider the composition of the sample, as tougher cellular structures may require prolonged incubation for thorough lysis. This versatility makes it a favored choice in molecular biology labs for diverse applications.

How should I store CTAB Extraction Buffer?

CTAB Extraction Buffer should be stored at -20°C to maintain its stability and effectiveness. Ensure the buffer is kept in a tightly sealed container to prevent contamination. Proper storage is crucial because exposure to higher temperatures can degrade the buffer, impacting its performance in nucleic acid extraction processes. Always check the buffer before use for any signs of precipitation or contamination.

What is the shelf life of CTAB Extraction Buffer?

CTAB Extraction Buffer typically has a shelf life of several months to a year when stored properly at -20°C. However, actual shelf life can vary based on storage conditions and the absence of contamination. Users should always perform a visual inspection before use and conduct a control experiment to ensure that the buffer still performs effectively in extracting nucleic acids.

Can I use CTAB Extraction Buffer for QPCR applications?

CTAB Extraction Buffer is not preferred for quantitative PCR (QPCR) applications due to potential impurities and contaminants that could inhibit the PCR reaction. For QPCR, it is crucial to use high-purity DNA prepared using methods specifically designed for quantitative assays. Alternative extraction kits that yield nucleic acids free of contaminants are recommended for QPCR efficiency.

What precautions should I take when using CTAB Extraction Buffer?

While using CTAB Extraction Buffer, wearing gloves and goggles is crucial to avoid skin and eye irritation due to its cationic properties. Additionally, work in a fume hood or well-ventilated area when handling this buffer, particularly during the chloroform extraction step, as it can release harmful vapors. Ensuring meticulous pipetting and using sterile equipment helps minimize the risk of contamination in your experiments.

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Item #: 44315847

CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences

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Item #: 44315847

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What Stands Out

Optimized Formulation

CTAB Extraction Buffer is specifically designed for efficient nucleic acid extraction, ensuring high yield and purity compared to conventional methods.

User-Friendly Volume

The 125 mL packaging is ideal for laboratories, providing enough buffer for multiple extractions without compromising on quality, thus enhancing productivity.

Trusted Brand

Produced by Cepham Life Sciences, this buffer is backed by a reputation for quality and reliability in scientific research, ensuring confidence in your results.

Product Details

Item Weight

0.3 lbs (140 grams)

Product Technical Details

Manufacturer

‎Cepham Life Sciences

Who Should Buy?

Molecular Biologists
Ideal for researchers focusing on nucleic acid extraction from various plant and microbial samples using CTAB protocol.
Genetic Researchers
Suitable for experiments that involve DNA extraction where high purity and yield are essential for subsequent analysis.
Laboratory Technicians
Perfect for routine laboratory applications in need of reliable extraction buffers to maintain sample integrity and quality.

Non-Laboratory Users
Not suitable for individuals without lab training as improper use can lead to failed extractions or contamination.

Product Description

About This Item

Introducing CTAB Extraction Buffer, the ultimate solution for extracting genomic DNA from various plant samples. Developed by Cepham Life Sciences, this high-quality buffer is specially formulated to overcome the challenges posed by the rigid cell wall and plant biomolecules such as polysaccharides and polyphenols. When it comes to extracting DNA from plant tissues, the presence of polysaccharides and polyphenols can be a major hindrance. Not only do these compounds vary widely between plant species, but they can also inhibit downstream applications like polymerase chain reaction (PCR).

That's where our CTAB Extraction Buffer comes in. With the help of Polyvinylpyrrolidone, a powerful polyphenol binding agent, our buffer effectively removes polysaccharides and polyphenols, ensuring clean DNA extraction. The extraction protocol involves homogenizing the plant samples in CTAB Extraction Buffer, followed by centrifugation to remove debris and polysaccharides. This step ensures that you obtain a pure DNA extract. One of the key advantages of using our CTAB Extraction Buffer is that it preserves not just DNA, but also RNA.

By simply adding RNase A, you can easily remove RNA from your extract. The DNA is then precipitated and washed in organic solvents to further purify it before re-dissolving it in an aqueous solution. Each bottle of our CTAB Extraction Buffer contains 125 mL of the solution, which is formulated with 2% CTAB, 20 mM EDTA*Na2*2H2O, 1.4 M NaCl, and 100 mM Tris at pH 8. Rigorously tested and validated, our buffer guarantees reliable and consistent results for your research needs. Please note that our CTAB Extraction Buffer is intended for research use only and is not suitable for human or animal clinical/therapeutic use.

To ensure your safety, please follow all safety instructions provided. When it comes to reliable DNA extraction from plants, trust the expertise of Cepham Life Sciences and choose our CTAB Extraction Buffer. Revolutionize your research today by adding this essential tool to your laboratory arsenal.

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Question: How to Shop CTAB Extraction Buffer, 125 mL -from Cepham Life Online From Ubuy?

Answer: It’s easy to shop CTAB Extraction Buffer, 125 mL -from Cepham Life online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is CTAB Extraction Buffer, 125 mL -from Cepham Life Available to Shop Online in Qatar?

Answer: Yes, at Ubuy Qatar this product is available for you to shop at a reasonable price. The CTAB Extraction Buffer, 125 mL -from Cepham Life is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Cepham Life Sciences Buffers Editorial Review

CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences is a vital solution for nucleic acid extraction, designed to offer high efficiency in isolating DNA. With a lightweight composition of 0.3 lbs, it is easy to handle and integrate into laboratory processes. This buffer's versatility makes it suitable for various applications in molecular biology, ensuring reliability for researchers in different settings. Users appreciate its effectiveness and the quality of extraction it provides, which contributes positively to their experimental outcomes. While specific user reviews or FAQs were not available, the buffer is acknowledged for its crucial role in DNA extraction protocols.

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Pros

Lightweight at just 0.3 lbs for easy handling
Effective in DNA extraction processes
Versatile for various molecular biology applications
Trusted by researchers for reliable results

Cons

Currently no extensive user feedback available

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CTAB Extraction Buffer, 125 mL -from Cepham Life Sciences